ABSTRACT
Pet rabbits have increased their popularity in a lot of countries. However, most of the laboratory profiles in rabbit medicine come from the observations made in rabbit as biomodels or meat production. So that further researches are necessary to obtain reference values for hematology and biochemical profiles in pet rabbits and the different breeds, especially, in relation to acid-base balance. The aim of this report was to offer the mean values of the main parameters connected with acid-base profile in Netherland Dwarf breed. Thirty-five healthy rabbits (15 males and 20 females) were studied. Venous blood sample from lateral saphenous vein was analyzed to measure: haematocrit, haemoglobin, blood urea nitrogen, glucose, blood pH, partial pressure of CO2 (pCO2), total CO2, ions bicarbonate, chloride, sodium, potassium, base excess and anion Gap. Results showed a shorter range that those reported by different researchers. Moreover, differences between genders were showed in pCO2, its values were higher in males. It may be associated with a greater cellular metabolism. Values obtained in this research should be taken into account by veterinary clinicians for this breed in their clinical assessments. Besides, these values provide new results in parameters with few reference values.
A popularidade de coelhos como animais de estimação aumentou em muitos países. No entanto, a maioria dos perfis de laboratório em medicina de coelhos advém das observações de biomodelos animais ou da produção de carne. Assim, são necessárias pesquisas adicionais para obter valores de referência para hematologia e perfis bioquímicos em coelhos de estimação, e das diferentes raças, especialmente, em relação ao equilíbrio ácido-base. O objetivo deste relatório foi oferecer os valores médios dos principais parâmetros ligados ao perfil ácido-base na raça Anã Holandês. Trinta e cinco coelhos saudáveis (15 machos e 20 fêmeas) foram estudados. A amostra de sangue venoso da veia safena lateral foi analisada para mensuração: hematócrito, hemoglobina, nitrogênio ureico sanguíneo, glicose, pH sanguíneo, pressão parcial de CO2 (pCO2), CO2 total, íons bicarbonato, cloreto, sódio, potássio, excesso de base e ânion Gap. Os resultados apresentaram um intervalo menor do que aqueles relatados por diferentes pesquisadores. Além disso, as diferenças entre os gêneros foram mostradas na pCO2, seus valores foram maiores no sexo masculino. Pode estar associado a um maior metabolismo celular. Os valores obtidos nesta pesquisa devem ser levados em consideração pelos clínicos veterinários para esta raça em suas avaliações clínicas. Além disso, esses valores fornecem novos resultados em parâmetros com poucos valores de referencia.
Subject(s)
Animals , Male , Female , Rabbits , Potassium/blood , Sodium/blood , Acid-Base Equilibrium , Pets/blood , Reference Values , Blood Specimen Collection/veterinaryABSTRACT
Abstract Pet rabbits have increased their popularity in a lot of countries. However, most of the laboratory profiles in rabbit medicine come from the observations made in rabbit as biomodels or meat production. So that further researches are necessary to obtain reference values for hematology and biochemical profiles in pet rabbits and the different breeds, especially, in relation to acid-base balance. The aim of this report was to offer the mean values of the main parameters connected with acid-base profile in Netherland Dwarf breed. Thirty-five healthy rabbits (15 males and 20 females) were studied. Venous blood sample from lateral saphenous vein was analyzed to measure: haematocrit, haemoglobin, blood urea nitrogen, glucose, blood pH, partial pressure of CO2 (pCO2), total CO2, ions bicarbonate, chloride, sodium, potassium, base excess and anion Gap. Results showed a shorter range that those reported by different researchers. Moreover, differences between genders were showed in pCO2, its values were higher in males. It may be associated with a greater cellular metabolism. Values obtained in this research should be taken into account by veterinary clinicians for this breed in their clinical assessments. Besides, these values provide new results in parameters with few reference values.
Resumo A popularidade de coelhos como animais de estimação aumentou em muitos países. No entanto, a maioria dos perfis de laboratório em medicina de coelhos advém das observações de biomodelos animais ou da produção de carne. Assim, são necessárias pesquisas adicionais para obter valores de referência para hematologia e perfis bioquímicos em coelhos de estimação, e das diferentes raças, especialmente, em relação ao equilíbrio ácido-base. O objetivo deste relatório foi oferecer os valores médios dos principais parâmetros ligados ao perfil ácido-base na raça Anã Holandês. Trinta e cinco coelhos saudáveis (15 machos e 20 fêmeas) foram estudados. A amostra de sangue venoso da veia safena lateral foi analisada para mensuração: hematócrito, hemoglobina, nitrogênio ureico sanguíneo, glicose, pH sanguíneo, pressão parcial de CO2 (pCO2), CO2 total, íons bicarbonato, cloreto, sódio, potássio, excesso de base e ânion Gap. Os resultados apresentaram um intervalo menor do que aqueles relatados por diferentes pesquisadores. Além disso, as diferenças entre os gêneros foram mostradas na pCO2, seus valores foram maiores no sexo masculino. Pode estar associado a um maior metabolismo celular. Os valores obtidos nesta pesquisa devem ser levados em consideração pelos clínicos veterinários para esta raça em suas avaliações clínicas. Além disso, esses valores fornecem novos resultados em parâmetros com poucos valores de referencia.
ABSTRACT
Objective To evaluate the performance portable analyzer and reagent disc kit for liver function tests .Methods Ac‐cording to EP10‐A2 document of Clinic and Laboratory Standards Institute ,specimens with high ,medium and low concentrations were measured continuously in a specific order for 5 days .The bias and the total imprecision were calculated ,and the intercept ,slope rate ,non linearity ,carryover contamination and drift were analyzed by using multiple regression analysis .Results The bias and pre‐cision of specimens with high ,medium and low concentrations were all within acceptable ranges .No significant difference was showed in intercept ,slope rate ,non linearity ,carryover contamination and drift(P>0 .05) .Conclusion Portable analyzer and rea‐gent disc kit for liver function tests could be with fine precision ,fine linearity ,low carryover contamination and good stability ,and could meet clinical application requirement .
ABSTRACT
OBJECTIVES: OCT(Point-of-Care Test), often translated as "Bedside laboratory", is a testing method used in most developed countries to conduct medical research. It is known to extract rapid results that can be applied in the intensive care unit. This study was conducted to investigate the correlation of the i-STAT(TM) POCT analyzer with the traditional test in the neonatal intensive care units. METHODS: 60 babies(birth weight > or =1.0kg) who were admitted to neonatal intensive care unit from June, 2000 to June, 2001 at Asan Medical Center requiring blood samples for the testing of arterial blood gas analysis(ABGA), electrolytes, hemoglobin(Hb), hematocrit(Hct), were included in the study. Blood samples were taken simultaneously to be tested by the traditional laboratory method and by i-STAT(TM) (i-STAT Co. USA) POCT analyzer. The data used for comparison analyzation included pH, pCO2, pO2, HCO3, Na, K, Hb, and Hct. RESULTS: 245 measurements of ABGA and 195 measurements of Na, K, Hb, Hct from 60 babies were used for comparison. Good correlation of data was found between i-STAT(TM) POCT analyzer and traditional laboratory method obtained from the central laboratory(correlation coefficient: pH 0.954, pCO2 0.944, pO2 0.941, HCO3 0.880, Na 0.713, K 0.860, Hb 0.864, Hct 0.880). CONCLUSION: This study showed that i-STAT(TM) POCT analyzer provided accurate analytic results when compared with traditional laboratory method used in the neonatal intensive care units.